Unlockable: Second Story Mode
To unlock the Second Story Mode, clear all one hundred floors on Story Mode.

Unlockable: Characters
To unlock the characters listed below, fulfill the requirement listed.

-Itachi - Clear thirty floors in Second Story Mode

-Jiraiya - Clear one hundred floors in Story Mode

-Kabuto - Clear one hundred floors in Story Mode

-Kisame - Clear thirty floors in Second Story Mode

-Orochimaru - Clear one hundred floors in Story Mode

-Sasuke - Clear one hundred floors in Story Mode

-Shizune - Clear one hundred floors in Story Mode

-Third Hokage - Clear thirty floors in Second Story Mode

-Tsunade - Clear one hundred floors in Story Mode

Hint: New Teams
Below are a list of new teams that appear in the game.

-Cunning Master and Student - Tsunade and Sakura

-Dark Alliance - Orochimaru, Kabuto, and Sasuke

-Hokage and Assistant - Tsunade and Shizune

-Leaf Jonin - Shizune, Guy, and Kakashi

-Shadows of Evil - Orochimaru and Kabuto

-The Determined Ones - Naruto and Sakura

-The Guides - The Third Hokage and Tsunade

-The Leaf's Greatest - Tsunade, Jiraiya, and The Third Hokage